Cause Thyroiditis Generates Neoantigenic Determinants That Iodination of Tyrosyls in Thyroglobulin

Iodination of Tyrosyls in Thyroglobulin

Iodine content of thyroglobulin in Nod.H2h4 mice developing iodine-accelerated autoimmune thyroiditis.

OBJECTIVE In NOD.H2h4 mice, high dietary iodine intake has been known to cause iodine-accelerated spontaneous autoimmune thyroiditis (ISAT) via an unknown mechanism. The aim of the study was to examine whether the NOD.H2h4 genetic background predisposes to enhanced iodine organification in thyroglobulin (Tg), a target autoantigen in ISAT. DESIGN To avoid issues associated with an ongoing anti...

Molecular parameters linking thyroglobulin iodination with autoimmune thyroiditis.

Increased iodide intake has been linked to the development of hypothyroidism and/or autoimmune thyroiditis in humans and animals, but the mechanisms involved remain poorly understood. Increased ingestion of iodide is likely to have pleiotropic effects on either metabolic or immunological processes. Within the latter domain, recent interest has been focused on two areas: a) the role of iodinated...

Defective thyroglobulin synthesis in an experimental rat thyroid tumor: iodination and thyroid hormone synthesis in isolated tumor thyroglobulin.

Purified rat tumor thyroglobulin from the experimental rat thyroid tumor, line 1-1C2, was studied for its thyroid hormone content after in vivo and in vitro iodination and compared with normal and desialylated normal rat thyroglobulin. Tumor thyroglobulin had a very low sialic acid and iodine content; after in vivo iodination it contained only small amounts of triiodothyronine (T3) and no detec...

Enzymatic iodination of tyrosine and thyroglobulin with chloroperoxidase.

1. Crystalline chloroperoxidase is effective in catalyzing the iodination of tyrosine or thyroglobulin when supplemented with HzOz, or with the H20z-generating system, glucoseglucose oxidase. The iodination reaction is very rapid at low concentrations of iodide (5 X 10-5 M), and at low concentrations of tyrosine (1 X 10V4 M) or thyroglobulin (0.33 mg per ml). 2. Iodide is rapidly bound as 3-iod...